Fth1 sirna

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August undefined, 2024

WebSep 21, 2024 · FTH1, a key subunit of ferritin, is involved in a variety of disease signaling pathways. In particular, the expression of FTH1 varies in different diseases. FTH1 was … WebOct 19, 2014 · Analysis of NCOA4 −/− cell extracts using SEC revealed that the faster-migrating FTH1 band was absent in all fractions, and that the relative amount of FTH1 and FTL was greater in fractions ...

FTH1 Inhibits Ferroptosis Through Ferritinophagy in the 6 …

WebFTH1. Product Clone Name. B-12. Classification. Primary Antibody. Clonality. Monoclonal Antibody. Antibody Isotype. IgG 2a kappa light chain. Amino Acids. 131-183. Epitope … WebDownload scientific diagram Zeta potential analysis of siRNA: liposome complexes used for GIC transfection. from publication: Liposomal delivery of ferritin heavy chain 1 (FTH1) siRNA in patient ... the margraves

DC-Chol/DOPE Blend powder Avanti Polar Lipids - Sigma-Aldrich

WebSep 6, 2024 · GICs were transfected with FTH1 or control siRNA at siRNA (μg): liposome (μl) ratios of 2:4 (T3691) or 2:8 (T387) for 24h, followed by no radiation (0Gy) or radiation at 4Gy and 8Gy. Cell viability was assessed 24 and 48 hours after radiation exposure using MTS assay. (A, B) In T3691 GICs, at both 24h (a) and 48h (b) post radiation, cell viability … WebGenetic locus: FTH1 (human) mapping to 11q12.3. PRODUCT ferritin heavy chain siRNA (h) is a pool of 3 target-specific 19-25 nt siRNAs designed to knock down gene expression. Each vial contains 3.3 nmol of lyophilized siRNA, sufficient for a 10 µM solution once resuspended using protocol below. Suitable for 50-100 transfections. WebSep 12, 2024 · We also observed a decrease in FTH1 with either of the two ISCA2 siRNAs at earlier time points that was not observed after 6 days’ siRNA transfection, which may suggest dynamic regulation of ... themarh

pAV-FTH1-shRNA Charles River Laboratories

Fth1 sirna

最新中国发明专利第2024-03-31期 - 专利汇 PatentHub专利检索

WebDec 2, 2024 · siRNA-mediated silencing of gene expression was performed with Lipofectamine 3000 per the manufacturer’s guidelines using control, prominin2, TSG101, … WebDec 1, 2024 · Subsequently, we showed that ferritin heavy chain 1 (FTH1) was a key determinant for baicalin-induced ferroptosis. Overexpression of FTH1 abrogated the …

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Web70% confluent, they were transfected by addition of 2μg of FTH1 siRNA (Santa Cruz) or con-trol silencer firefly luciferase siRNA (ThermoFisher) complexed with liposomes in a 2μg:4 μl ratio (siRNA: liposome) for T3691 cells and 2μg:8μl ratio for T387 cells. Cells were incubated with complexes for the duration of the experiment. WebMar 28, 2024 · A non-targeting control siRNA pool (Horizon Discovery, D-001810-10-05), human FTH1 siRNA pool (Horizon Discovery, L-019634-00-0005), human HNRNPA2B1 siRNA pool (Horizon Discovery, L-011690-01-0005 ...

WebCatalog number: SH884970. Description: Four pre-designed shRNA constructs targeting ferritin, heavy polypeptide 1 (FTH1) and one scrambled control. Each shRNA construct is … WebMay 19, 2024 · Elevated FTH1 mRNA levels were correlated with worse prognosis of RCC patients. Cox multivariate survival analysis indicated that age, stage, and M stage were …

Web39 FTH1 Silencer Select Pre-designed, Validated, and Custom siRNA in Standard, HPLC, and In-vivo Ready Purities. Web70% confluent, they were transfected by addition of 2μg of FTH1 siRNA (Santa Cruz) or con-trol silencer firefly luciferase siRNA (ThermoFisher) complexed with liposomes in a …

WebSimilar to the T3691 cells, T387 cells showed a significant loss of cell viability following transfection with FTH1 siRNA at 0Gy (57%) (Fig 4 C), but 24h post radiation exposure at 4Gy (64%) and ...

WebFeb 22, 2024 · Doxorubicin (DOX) is an anthracycline antibiotic that is used extensively for the management of carcinoma; however, its clinical application is limited due to its serious cardiotoxic side effects. Ferroptosis represents iron-dependent and reactive oxygen species (ROS)-related cell death and has been proven to contribute to the progression of DOX … the margrave preyWebApr 3, 2024 · To further examine whether iron overload is caused by ferritinophagy, we assessed the effect of Sch B on the expression of FTH1 in cultured LX2 cells. The protein expression of FTH1 was increased by Sch B (Figure 4(e)). Additionally, Sch B promoted the binding of NCOA4 to FTH1 in vitro (Figure 4(f)). Collectively, these data suggested that … the margret mcbride literary agencyWebMar 21, 2024 · FTH1 (Ferritin Heavy Chain 1) is a Protein Coding gene. Diseases associated with FTH1 include Hemochromatosis, Type 5 and Macular Dystrophy, … the margy songWebNov 12, 2015 · In addition, HCT116 cells sequentially transfected with a plasmid expressing an siRNA-resistant form of ISCU2 and siISCU showed higher expression of FTH1 . These findings indicated that ISCU2 ... the margsWeb特别声明：本产品及我公司所售其他产品均为科研类试剂产品，严禁用于药物、医疗及其他非科研用途。重组小鼠CD5 是高品质的分子生物学试剂产品，由北京百奥莱博供应，用于生化研究，本产品质量好，且极具价格优势，深为用户称道，了解更多重组小鼠CD5等分子生物学试剂产品请联系我司咨询 ... the mar hamlinWebin the preparation of liposomes for ferritin heavy chain 1 (FTH1) siRNA (small interference RNA) transfection studies ... (30:70, w/w) helps in transfection of therapeutic small interference RNA (siRNA). DC-Chol/DOPE liposomes are cationic, unilamellar and spherical in nature. It aids in improved condensation of DNA for transfection studies ... themar heightsWebNational Center for Biotechnology Information the margulies collection