H69 cholangiocyte cell line

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August undefined, 2024

Webhuman sclc cell lines nci h69 - by Bioz Stars , 2024-01 94 / 100 stars Images 1) Product Images from "Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines" Article Title: Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines Journal: BMC Cancer

Development and characterization of cholangioids from normal …

WebNational Center for Biotechnology Information WebDec 13, 2024 · H69 cells, an SV40-transformed (i.e., immortalized) human cholangiocyte cell line , were grown in DMEM Nutrient Mixture F-12 (DMEM/F-12) + GlutaMax supplemented with 10% FBS and 100 U/mL penicillin-streptomycin. RAW264.7 mouse macrophages were from the American Type Culture Collection (ATCC) and were … thornhill plumbing copperas cove

Establishment of a highly differentiated immortalized human ...

WebWe used the human cholangiocyte cell line (H69) and assessed it for expression and function of an a... View. Cathepsins as effector proteases in hepatocyte apoptosis. Article. Feb 1999; Web(Fig. 3A). This was further confirmed by western blots with clear bands indicating CD1d expression (Fig. 3B). The cholangiocyte phenotype of the H69 cell line was veri- fied … WebMay 1, 2024 · Cell lines. The cholangiocyte cell line H69 is a SV40-transformed bile duct epithelial cell line derived from a non-cancerous human liver [23]. H69 cells were … unable to locate package httpd

Infection with CagA+ Helicobacter pylori induces epithelial to ...

Establishment of a highly differentiated immortalized …

WebOct 11, 2024 · The H69 human cholangiocyte cell line was provided by Dr. Nicholas LaRusso (Mayo Clinic, Rochester, MN). Cells were grown in Dulbecco’s modified Eagle’s medium/F12 supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, adenine, insulin, epinephrine, T3-T, hydrocortisone, and epidermal growth factor. ... WebThis cell line is aneuploid, will form colonies in soft agar and retains small cell carcinoma morphology and ultrastructure as well as APUD cell characteristics. The cells grow in aggregates, thus cell counts are not accurate. The cells stain positively for cytokeratins. … thornhill police serviceWebThe human cholangiocytes (H69), provided by Dr. Douglas Jefferson (New England Medical Center, Tufts University, Boston, MA), were cultured as described. Infection of Cells and Inhibitors. RRV infection of cell lines was performed in 24‐well culture plates containing cells seeded at 4 × 10 5 /well and incubated at 37°C for 3 days. Confluent ... thornhill police

"WebH69, a human immortalized cholangiocyte cell line and KMCH, Mz-ChA-1, and HuCCT-1, human cholangiocarcinoma cell lines were grown as described. 11. BDEneu cells were a kind gift from Dr. Alphonse Sirica (Virginia Common-wealth University) and were grown as described. 12. Cells were treated with the indicated concentrations of FFAs (400-800 . μM) " - H69 cholangiocyte cell line

H69 cholangiocyte cell line

WebThe type III isoform of the inositol 1,4,5-trisphosphate receptor (InsP3R3) is apically localized and triggers Ca2+ waves and secretion in a number of polarized epithelia. However, nothing is known about epigenetic regulation of this InsP3R isoform. We investigated miRNA regulation of InsP3R3 in primary bile duct epithelia (cholangiocytes) … WebApr 28, 2024 · human cholangiocyte cell line, H69, a CCA cell line CC-LP-1, CagA + ve Helicobacter pylori, 40. and H. bilis bacilli. Exposure to increasing numbers of H. pylori at 0, 1, 10,100 bacteria per . 41.

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WebApr 9, 2014 · In addition, we showed that cholangiocyte-like cells could also be generated from human induced pluripotent stem cells, … WebIn this study, cytotoxicity assays were employed to establish the toxicity profiles for 24 hr exposure of lung cancer cell lines, A549 and Calu-1, to the commercially available drug, doxorubicin (DOX). Raman spectroscopy, coupled with Confocal Laser Scanning Microscopy and Flow Cytometry, was used to track the DOX mechanism of action, at a ...

WebApr 13, 2015 · Furthermore, the number (iDC: 7941, H69: 9724, NHC: 9295) and magnitude (Supplementary Figure 4) of differentially expressed genes are ultimately similar to both cholangiocyte cell lines. WebJul 24, 2024 · The human cholangiocyte H69 cell line is a SV40-transformed human bile duct epithelial cell line derived from a normal liver 61. The H69 cells were used between passages 10 and 30 and grown in H69 medium that included 43.82% DMEM medium (Dulbecco’s Modified Eagle’s Medium), 43.82% DMEM/Ham’s F12, 10% fetal bovine …

WebSep 11, 2024 · The normal human cholangiocyte cell line (NHC) was isolated from a normal human liver and characterized by Joplin et al 17 and was a gift from Dr Juan Medina (University of Navarra, Pamplona ... WebOct 12, 2024 · Here, exosome-mediated transfer of mRNAs from the human cholangiocyte cell line (H69) was investigated following exposure to Ov- GRN-1, to naïve recipient H69 cells. To minimize the influence of endogenous huPGRN, the gene encoding huPGRN was inactivated using CRISPR/Cas9-based gene knock-out.

WebDec 3, 2015 · Cell lines. H69 cells (a gift of Dr. D. Jefferson, Tufts University) are SV40 transformed normal human biliary epithelial cells originally derived from liver harvested for transplant.

WebMay 15, 1999 · In summary, the human cholangiocyte cell line H69 possesses a functional bile acid transporter, and GUDC decreases BV-induced apoptosis and inhibits activity of caspase 3 protease by blocking CC release from mitochondria. unable to locate package inetutils-pingWebProduct Description. ". Human Cholangiocyte Primary Cell Culture - Frozen Vial. Source: Human Biliary Epithelial Cells. Positive Markers: CK7, CK19, glutamyl transpeptidase, … thornhill plumbing and heating cardiffWebCell Culture. H69 cells, immortalized human cholangiocytes, were obtained from Dr. Doug Jefferson (Tufts University, Boston, MA) and cultured as previously described.16 For … thornhill pool addressWebJun 1, 2004 · The cholangiocyte cell line H69 is a SV40-transformed bile duct epithelial cell line derived from a non-cancerous human liver [53] and was obtained in 2010 from Dr. Gregory J. Gores, Mayo Clinic ... unable to locate package ia32-libs-gtkWebCholangiocyte cell line. Cholangiocytes are epithelial cells which line the bile ducts. Please note that there is a distinct lung carcinoma cell line with the same name (H69). … unable to locate package iftopWebWe established an in vitro human model using an immortalized human cholangiocyte cell line and primary human cholangiocytes obtained from explanted livers to determine human cholangiocyte susceptibility to rotavirus infection. unable to locate package ibus-pinyinWebSep 22, 2024 · Basel, Switzerland, 22 September 2024 – Lonza, a global manufacturing partner to the pharma, biotech and nutrition industries, today announced it will invest in … thornhill police station contact details