Linear puromycin marker

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Nettet27. mai 2024 · In some cases, addition of a Kozak consensus ribosome binding site (4) may improve expression levels; however, many cDNAs have been efficiently expressed in Tet systems without the addition of a Kozak sequence. pTRE-Tight-Gene X plasmids should be cotransfected with the Linear Hygromycin Marker (#631625 or #6202-1, not … Nettet13. okt. 2015 · pTRE3G, pTRE3G-Luc and Linear Puromycin Marker were pur-chased from Clontech Laboratories, Inc (Mountain V iew, CA, USA). The plasmid pTRE-p53 was constructed by inserting full-length human p53 ...

Selection marker (Linear / Plasmid) - takara.co.kr

Nettet25. jan. 2014 · We test a puromycin- N -acetyltransferase gene ( pac) as a selection marker in ciliates. The pac gene was altered to the most preferred codon-usage in T. … Nettet30. mar. 2024 · Similarly, we transfected the pX330-U6-Chimeric_BBCBh-hSpCas9 empty vector into HEC-1B and AN3CA cells with a linear puromycin marker and constructed a stable cell line, which was used as a ... birch lane salinas writing desk

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NettetSuccessful stable transfection requires both effective DNA delivery and a way to select cells that have acquired the DNA. Approximately 1 in 10 4 transfected cells will stably … NettetLinear selection markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer linear selection markers for either hygromycin (Line ... NettetLinear Puromycin Marker (50 ng/µl) (also sold separately as Cat. No. 631626) 100 rxns Xfect™ Transfection Reagent (Cat. No. 631317) − 2 tubes − 2 tubes Xfect Polymer (75 … birch lane ratings

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Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb marker is compatible with any expression vector (including Tet System vectors) for generating stable transfectants using CalPhos transfection reagent or a ... Nettet28. aug. 2024 · 上海市闵行区动物疫病预防控制中心，上海201109) 摘要:应用Tet-on 系统和H1299细胞，建立了p53诱导表达的H1299细胞系p53-Tet H1299 , p53表达受强力霉素 ( doxycycline. Dox) 调控，存在一定的剂量依赖性诱导表达的p53 蛋白有很好的核定位能力和转录活性，能显著上调p21 ... birch lane phone numberNettetcDNA was transfected into A549 cells with linear puromycin marker (Clontech). Puromycin-resistant clones were selected as Tet-Express inducible cells. NR0B1 expression induced by the transfection of Tet-Express with Xfect transfection reagent (Clontech) was confirmed by immunoblotting as described below. birch lane schaffer extendable dining table

"NettetLinear selection markers are ideal for cotransfection with any expression vector where stable integration and expression are required. We offer linear selection markers for … " - Linear puromycin marker

Linear puromycin marker

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NettetPlasmid vectors using puromycin resistance as a selective marker have been developed for many methanogenic Archaea. The current vectors are constructed either with … Nettet9. jan. 2024 · Linear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly-adenylation signal. The 1.8-kb …

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NettetSelection markers are common genetic elements used in recombinant cell line development. ... puromycin, and Zeocin™, and their corresponding selector genes, using a green fluorescent protein (GFP) as a reporter in two model cell lines, HT1080 and HEK293. We identify Zeocin™ as the best selection agent for cell line development in … NettetLinear Puromycin Marker, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, …

NettetLinear Puromycin Marker HindIII & XbaI 0.45, 0.6, & 0.75 kb Functional Testing of Linear Markers HEK 293 cells were transfected with 200 ng of either the Linear Hygromycin … Nettet联系人：Dr.Xu, Biovector NTCC Inc. 电话： 400-800-2947 工作QQ:1843439339 (微信同号) 邮件： [email protected]. 手机： 18901268599. 地址：北京. 已注册. 详细信息. 咨询记录 (共 0 条) Linear Puromycin Marker.

NettetLinear Hygromycin Marker : 2 μg: 319,000원 Clontech: 631626: Linear Puromycin Marker : 2 μg: 319,000원 Clontech: 631601: pPUR Vector : 25 μg: 751,000 ... (Table I). … NettetLinear Puromycin Marker. 2 μg. 319,000원. pTRE-Tight는 response plasmid로 최적화된 TRE 기반의 promoter인 PTight를 가지고 있어 정밀하게 목적 유전자의 유도 발현을 조절할 수 있다 (Figure 1). PTight는 낮은 basal expression과 최고의 inducible expression을 제공한다. 또한, pTRE-Tight와 Linear ...

NettetManufacturer: Clontech Labs 3P 631626. Linear Puromycin Marker, Size: 2microgram, it is a short ds DNA fragment consisting of the puromycin resistance gene regulated by …

NettetPlate your Tet-On Advanced cell line at a density appropriate for your preferred transfection method. 2. Combine your customized pTRE-Tight vector and either the Linear Hygromycin or Puromycin Marker at a ratio of 20:1 (i.e., 20-fold less linear marker), and transfect the Tet-On Advanced cells using your preferred method. dallas hair salon owner shelley luther dallas half marathon courseNettetLinear Puromycin Marker is a short ds DNA fragment consisting of the puromycin resistance gene regulated by the SV40 promoter and ending with the SV40 poly … birch lane recliner chairNettet13. okt. 2015 · Colonies were picked and tested by luciferase assay using pTRE3G-Luc transfection and Dox induction. The G418-resistant colony with the highest fold induction was propagated and transfected with plasmid pTRE-p53 and puromycin linear selection marker. Transfectants were selected with 1 μg ml −1 puromycin for 2 weeks. dallas halloween block party 2023Nettet631626 - Linear Puromycin Marker Überarbeitet am 2024-12-23 1-800-633-8253 (US) or 801-629-0667 (international). Italien Marco Marano CAV “Osp. Pediatrico Bambino … dallas hammer divinityNettet4. okt. 2024 · The puromycin-resistant gene region, amplified from the linear puro marker (Cat. No. 631626, Takara-Bio), was inserted using the In-fusion HD cloning kit (Cat. No. 639648, Takara-bio) ... After a puromycin selection, cells expressed each BioID-tagged gene by doxycycline with biotin. BioID alone or BioID-tagged Luc2 ... birch lane reviews and complaintsNettet美国Clontech公司是一家专门致力于基因工程及蛋白质工程领域研究用产品的研发和生产的专业公司。公司产品涉及PCR产品、RT－PCR产品、cDNA文库构建、基因克隆、基因表达、基因分析、蛋白质技术等诸多领域。产品种类多达2000多种。Clontech公司拥有多种独立知识产权产品， birch lane same as wayfair