WebJan 4, 2024 · Of the candidate genes, we have recently reported an ethylene-responsive element binding factor, PhERF2, which plays a critical role in TRV-induced RNA silencing and antiviral defense via transcriptional modulation of RDR2, RDR6, DCL2, and AGO2 in petunia ( Sun et al., 2016 ). Dual luciferase assay was performed using a method previously described41. The full-length coding sequence of PhERF2 was amplified and inserted into pGreenII62-SK vector as the effector, driven by CaMV 35S promoter, and the empty vector was used as a control. To generate the reporter construct, a 1256-bp ADH1 … See more Petunia (Petunia × hybrida) cultivar ‘Mitchell Diploid’ was used for generation of stable transformants. Seeds of wild-type (WT) and homozygous T2 generation transgenic petunia … See more To examine the impact of waterlogging stress on WT, PhERF2-RNAi, and -overexpressing plants, the following treatments were applied to 5-week-old petunia plants before … See more A 339-bp fragment and a full length 1143-bp fragment harboring the ORF region of PhERF2 were PCR-amplified and cloned into pGSA1285 and pGSA1403 vectors for generating the RNAi … See more Total chlorophyll (a + b) content was measured as described previously37. Briefly, fresh leaf samples (0.1 g) were extracted for 24 h … See more

A petunia ethylene-responsive element binding factor, …

WebApr 12, 2024 · 2.5 Molecular dynamic simulations. Molecular dynamics (MD) simulation is a technique that can be used effectively to understand macromolecular structure-to-function relationships (Ahmad & Kesavan, 2024).MD simulation is most widely used to evaluate the stability and to enhance low quality models (Sokkar et al., 2011).Here, it was used to … WebCN110736811A CN202411138329.3A CN202411138329A CN110736811A CN 110736811 A CN110736811 A CN 110736811A CN 202411138329 A CN202411138329 A CN 202411138329A CN 110736811 A CN110736811 A CN 110736811A Authority CN China Prior art keywords stage chlorophyll petunia pherf2 malondialdehyde Prior art date 2024 … lindsey lepley

PhERF2, an ethylene-responsive element binding factor, plays an ...

WebApr 19, 2016 · Europe PMC is an archive of life sciences journal literature. WebJul 1, 2024 · Ethylene-responsive element binding factors (ERFs) are involved in regulation of various stress responses in plants, but their biological functions in waterlogging stress … WebThe invention discloses transgenic lines of petunia PhERF2 and a wild type waterlogging tolerance identification method thereof, which comprises the steps of S1, carrying out pot flooding identification on the transgenic lines of the petunia PhERF2 and the wild type thereof, enabling the transgenic lines to flood 2-3cm above the ground surface, … lindsey leigh twitter