S5a protein

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August undefined, 2024

WebFeb 19, 2005 · Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. … WebDownload scientific diagram DNA and protein sequence of S5a. The sequence depicted encodes the entire S5a protein beginning with potential initiation Met codons at positions …

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WebThus, the S5a protein provides a general model for studying recognition of polyubiquitin chains by a 26 S protease component. S5a retains the ability to bind ubiquitin chains even after it has been separated on a SDS-acrylamide gel and transferred to nitrocellulose. WebS5a is one of the integral Ub receptors of the 26S proteasome. It’s N-terminal vWA domain interacts with Rpn9 and Rpn10 on the 26S proteasome. The C-terminal domain of S5a … david and goliath chapter 4

The ubiquitin-interacting motif protein, S5a, is

WebS5a/PSMD4 Antibody detects endogenous levels of total S5a/PSMD4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are … WebJun 15, 2015 · The HIS-tagged human S5a protein was expressed in bacteria following cloning of the entire open reading frame (377 aa) in the pET-21a (Novagen, Billerica, MA) … david and goliath cigars for sale

Recombinant Human S5a/Angiocidin Biotin Protein, CF

DNA and protein sequence of S5a. The sequence depicted …

WebJul 7, 2013 · S5a/Rpn10 drives dendrite growth and elaboration in primary neurons and in the rodent brain in vivo We characterized the function of the key proteasomal lid-associated protein S5a/Rpn10 in the mammalian brain, utilizing granule neurons of the developing rat cerebellar cortex. WebJul 27, 2007 · Monoubiquitinated Pax3 was shuttled to the intrinsic proteasomal protein S5a by interacting specifically with the ubiquitin-binding protein Rad23B. Functionally, sustained expression of Pax3 proteins inhibited myogenic differentiation, demonstrating that Pax3 degradation is an essential step for the progression of the myogenic program. gas company in sierra vista azWebS5a/Angiocidin, also known as Anti-secretory Factor (ASF), is classified under the gene PSMD4, but is often referred to by a different name depending on the context in which it is … david and goliath children\u0027s story powerpoint

"WebS5a/Rpn10 is a major Ub-binding protein that binds preferentially to poly-Ub chains ( 13 ). It is found as a subunit of the 26S proteasome, but unlike other proteasome subunits, S5a exists predominantly as a free protein in the cytosol ( i.e. not incorporated into the proteasome) ( 14 , 15 ). " - S5a protein

S5a protein

Anti-Proteasome 19S S5A/ASF antibody (ab18512) Abcam

WebApr 10, 2024 · State Key Laboratory of Proteomics, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, 100850 P. R. China. Search for more papers by this author. ... (Figure 5J and Figure S5A,B, Supporting Information). Figure 5. Open in figure viewer PowerPoint. WebSep 13, 2006 · The results suggest that the protein folding reaction is linked to topology only in the sense that the native-state topology determines the repertoire of accessible …

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WebOct 14, 2013 · S5a is required for the degradation of p53 but not its E3 ligase Mdm2. Additional work is required to completely define the pathways of proteasomal recognition … WebS5a and ASF have identical 377 amino acid (aa) sequences, while Angiocidin is described as having an additional Gly255Glu256Arg257 sequence in its C-Terminus (1-3). The human …

WebKey features and details Mouse monoclonal [AH1.1] to Proteasome 19S S5A/ASF Suitable for: Flow Cyt, IP Reacts with: Human Isotype: IgG1 You may also be interested in Primary … WebOct 13, 2003 · The Binding Surfaces of S5a and the UBA Domains Overlap. We used an S5a construct spanning residues M196 to D307 to study the effect of S5a-binding on hHR23a protein structure. S5a (196–307) contains the two ubiquitin-binding domains and was previously shown to bind hHR23a with equal affinity to full-length S5a .

WebApr 12, 2024 · The gamma-hemolysin protein is one of the most common pore-forming toxins expressed by the pathogenic bacterium Staphylococcus aureus. The toxin is used by the pathogen to escape the immune system of the host organism, by assembling into octameric transmembrane pores on the surface of the target immune cell and leading to … WebS5a/Rpn10, a UIM-protein, as a universal substrate for ubiquitination The assay of the activity of ubiquitin (Ub) ligases (E3s) and screens for pharmacological agents that alter …

WebJun 15, 2015 · The HIS-tagged human S5a protein was expressed in bacteria following cloning of the entire open reading frame (377 aa) in the pET-21a (Novagen, Billerica, MA) vector. The plasmids were transformed into E. coli BL21 (DE3) and protein expression was induced with 1.0 mM IPTG for 4 hrs at 37°C.

WebApr 11, 2024 · Fabp5 is one kind of fatty acid binding protein that responds to chemical-induced skin inflammation and tumorigenesis [32]. Cd74, the macrophage migration inhibitory ... (Fig. S5A), therefore we further investigated the heterogeneity and transcriptional profiles of PBC in the AEW model. We re-analyzed PBC and found that they … david and goliath children storyWebFeb 19, 2005 · Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ... gas company in richmond vaWebS5a/PSMD4 Antibody detects endogenous levels of total S5a/PSMD4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly344 of human S5a/PSMD4 protein. david and goliath chapter 6 summaryWebThe human protein shares 96% and 99% aa sequence identity with its mouse and rat orthologs, respectively. Structurally, it contains an N-terminal von Willebrand Factor type A domain and two C-terminal Ubiquitin-interacting motifs (UIM). It acts as a Ubiquitin-binding protein where it is most commonly referred to as S5a or in yeast as Rpn10. david and goliath chapter 8 summaryWebProtein names Recommended name 26S proteasome non-ATPase regulatory subunit 4 Alternative names 26S proteasome regulatory subunit RPN10 26S proteasome regulatory subunit S5A Antisecretory factor 1 ( AF; ASF) Multiubiquitin chain-binding protein Gene names Name PSMD4 Synonyms MCB1 Organism names Organism Homo sapiens … gas company in shelbyville kyWebApr 11, 2003 · Total Structure Weight: 9.52 kDa Atom Count: 668 Modelled Residue Count: 85 Deposited Residue Count: 85 Unique protein chains: 1 Display Files Download Files 1P1A NMR structure of ubiquitin-like domain of hHR23B PDB DOI: 10.2210/pdb1P1A/pdb Classification: DNA BINDING PROTEIN Organism (s): Homo sapiens Expression System: … gas company in scott city ksWebS5A antibody; S5a/antisecretory factor protein antibody; see all. Images. Western blot - Anti-Proteasome 19S S5A/ASF antibody (ab18512) Crude Drosophila extract. Secondary antibody: rabbit polyclonal 1:5000. Please note: Drosophila S5a has an actual mass of 42.6 kDa, however, on the gel it runs at an apparent 54 kDa band. gas company in south carolina