WebTo overcome these challenges, we developed Topographic Single Cell Sequencing (TSCS) to measure genomic copy number profiles of single tumor cells while preserving their spatial context in tissue sections. We applied TSCS to 1,293 single cells from 10 synchronous patients with both DCIS and IDC regions in addition to exome sequencing. WebJan 4, 2024 · This hinders single-cell sequencing, because it contaminates samples with microbial species that distract the polymerases used in whole-genome amplification. …
Single-cell sequencing and its applications in head and neck cancer
WebJan 18, 2024 · Using a similar strategy, topographic single cell sequencing (TSCS) is designed to capture the genomic copy number profile of single tumour cells spatially. 17 By utilising TSCS in breast cancer samples, the results show a direct genomic lineage of breast cancer cell progression. Interestingly, the authors observe that most mutations and copy ... WebApr 13, 2024 · By developing a spatially resolved scDNA-seq method called Topographic Single Cell Sequencing (TSCS), genomic CNAs were linked to spatial information in ten cases of synchronous DCIS-IDC tissues, which showed that multiple clones co-migrated from the ducts into the invasive regions (Casasent et al., 2024). easy chair yoga for the elderly
Dissecting Cancer Development
WebJan 4, 2024 · The discovery was made possible by the researchers' development of a new analytical tool called topographic single cell sequencing (TSCS). WebJun 1, 2024 · Topographic single cell sequencing (TSCS, scDNA sequencing) On average 129 cells per patient: Chung et al. Nature Comm 2024: Park: Human: Cancer: 11x tumour cells from different breast cancer subtypes: Both: Dead cells were removed using Ficoll-Paque PLUS: scATAC-seq using the Fluidigm C1 platform: 515 cells: Chung et al. Cell … WebApr 1, 2024 · By developing a spatially resolved scDNA-seq method called Topographic Single Cell Sequencing (TSCS), genomic copy number aberrations (CNAs) were linked to spatial information in 10 cases of synchronous DCIS-IDC tissues, which showed that multiple clones co-migrated from the ducts into the invasive regions (Casasent et al., 2024). easychamber child